README for Figure S3.csv 
*** This file contains the raw data obtained on DNA and DNA-Rok complexes using microscale thermophoresis experiments represented in Figure S3 of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es


Legend Figure S3: MgCl2 does not affect the DNA binding affinity of Rok Kd-values (mM) obtained from fitting protein 
titration data to the McGhee-von Hippel equation as a function of MgCl2 concentration. The DNA used was 78 bp in 
length with or without a specific Rok binding site and the Rok concentration was varied between 0.125 and 16 M. 
The final measurement buffer consisted of 10 mM Tris HCl pH 8, 150 mM KCl, 5% glycerol, 0.05% Tween20 and 0.08 
mg/ml acetylated BSA. MgCl2 was added accordingly. Each data point was measured at least in triplicate and error 
bars represent the standard deviation. Some error bars are hidden behind the data points due to their small size. : 

*** The data were obtained using microscale thermophoresis experiments as described in the associated article. 

***The data are labeled by type of DNA used (Aspecific/Specific) and mM MgCl2 as represented in figure S3.
Column A: Amount of Rok in micromolar
Column B: Fnorm values replicate 1
Column C: Fnorm values replicate 2
Column D: Fnorm values replicate 3
Column E: Fnorm values replicate 4










